Blog > Fermentation #2 – Temperature Find A Store Yeast Experiment: Fermentation #2 – Temperature QUESTION WHAT EFFECT DOES TEMPERATURE HAVE ON THE FERMENTATION OF YEAST? MATERIALS 2 empty, clean 1-litre soda bottles 2 latex balloons 2 rubber bands Glass measuring cup, 1-cup capacity Measuring spoon: teaspoon Measuring spoon: tablespoon All-purpose flour Granulated sugar Water, room temperature 2 packets of Active Dry Yeast (each packet is ¼-ounces or 2¼ teaspoons) PROCEDURE Fill each soda bottle with one 1/4 ounce packet of Active Dry Yeast, 1 teaspoon sugar, 2 tablespoons all-purpose flour and 1 cup of room temperature water. Set one bottle in a vessel with warm water. Set the other bottle in a vessel with ice water. Secure a balloon on top of each bottle with a rubber band. Observe and record results. Keep both containers at a constant temperature. OBSERVATIONS & CONCLUSION 1. What effect does temperature have on the fermentation of yeast? 2. When was the difference noticeable?